Banca de DEFESA: CARLOS ALEXANDRE ROCHA DA COSTA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CARLOS ALEXANDRE ROCHA DA COSTA
DATA : 28/02/2020
HORA: 14:30
LOCAL: ITV
TÍTULO:

PROTEOMICS OF Isoetes cangae IN ALTERNATIVE AND NATURAL SUBSTRATES.

PALAVRAS-CHAVES:

Canga, stress, proteomics

PÁGINAS: 38
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Ferruginous rock fields or canga is an ecosystem that presents adverse edaphoclimatic conditions and high levels of endemism. For the mining industry, iron ore present in canga is of great interest for its economic potential, but this activity has a great environmental impact. Even with environmental protection measures such as recovery programs for degraded areas, for example, the activity severely affects local biodiversity. A recently discovered plant species called Isoetes cangae occurs exclusively submerged in a permanent natural lake in the Serra de Carajás, south of Pará. This species has been classified as critically endangered by international parameters. In the present study, I. cangae proteomics was performed in 3 alternative treatments and 1 treatment in natural soil, aiming to identify and quantify stress response-related proteins. We identified 638 proteins grouped into 321 clusters, where 112 clusters showed differential protein accumulation between treatments. The α-mannosidase protein was overexpressed on the three alternative substrates in relation to the control. Other proteins such as peroxidases, proteins in photosynthesis, proteins related to energy metabolism and repair and defense mechanisms were related to abiotic stresses, and salt stress is the most likely to occur in alternative substrates to justify the molecular changes that occurred. in I.cangae. Monitoring α-mannosidase expression may be a tool for identifying any adverse conditions for I.cangae, and finding favorable conditions for ex situ transplantation or environmental protection areas in order to preserve and propagate this species.

MEMBROS DA BANCA:
Externo à Instituição - AGENOR VALADARES SANTOS - UFPA
Externo à Instituição - GISELE FATIMA MORAIS NUNES - USP
Externo à Instituição - MARCELO MURAD MAGALHÃES - EMBRAPA
Presidente - 060.660.366-29 - RAFAEL BORGES DA SILVA VALADARES - UFRA
Interno - 644.120.402-91 - ROBERTO LISBOA CUNHA - UFRA