Banca de QUALIFICAÇÃO: DÁRCIA GABRIELA BORCEM DA COSTA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : DÁRCIA GABRIELA BORCEM DA COSTA
DATE: 04/08/2023
TIME: 14:00
LOCAL: ISARH, sala B
TITLE:

Development of microsatellite markers for Leiarius marmoratus as a strategy for genetic distance analysis and tool for Animal Breeding

KEY WORDS:

Catfish; Phylogeny; Molecular markers; NGS.

PAGES: 20
BIG AREA: Ciências Biológicas
AREA: Genética
SUBÁREA: Genética Animal
SUMMARY:

The Leiarius marmoratus (GILL, 1870), popularly known as “jundiá-da-amazônia”, presents great aquaculture potential in genetic improvement programs to increase zootechnical performance related to hybrid vigor. Fish farming stations have commercially exploited the hybrid “Pintado-da-Amazônia” obtained by crossing female Pseudoplatystoma punctifer x male Leiarius marmoratus. Thus, investigations of genetic relationships are crucial for further exploitation of L. marmoratus in genetic improvement programs. For that reason, the objective is to develop microsatellite markers for L. marmoratus with the purpose of phylogenetic characterization between different species of fish. Initially, a high molecular weight DNA sample will be submitted to next generation sequencing by the Illumina platform, using the MiSeq^TM and HiSeq^TM systems. The sequencing product will be evaluated by the FastQc program with respect to the parameters of total average quality per base, average quality per read and GC content of the sequences. Data filtering will be performed for base quality control and adapter removal, based on a minimum 20 Phred score. The genome de novo assembly will be tested in the SOAPdenovo2 and SPADES programs, whose quality of the generated products will be verified by the QUAST program. To identify the microsatellite markers, the MISA software will be used with selection parameters from 1 to 6 repetition motifs. The phylogenetic analysis will be carried out from the formation of outgroups, using data from all fish species available in the MicroSatellite DataBase repository to generate phylogenetic trees by Maximum Likelihood, Maximum Parsimony and Neighbor Joining methods, using the MEGA-X program with visualization and graphic edition in FigTree v.1.4. Therefore, it is expected to obtain a list of shared microsatellite markers between L. marmoratus and other fish species, as well as a panel of microsatellite markers specific to the species. Generating, therefore, tools for the exploration of genetic resources and application in genetic improvement programs of the native species.

BANKING MEMBERS:
Presidente - 1957010 - MARILIA DANYELLE NUNES RODRIGUES
Interno - 1312697 - MARCUS DE BARROS BRAGA
Externa ao Programa - 3346393 - ANA PATRICIA BARROS CORDEIRO
Externa à Instituição - REGIANNE MACIEL DOS SANTOS CORREA