Banca de DEFESA: JULIANA VASCONCELOS FIGUEIREDO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JULIANA VASCONCELOS FIGUEIREDO
DATE: 21/02/2025
TIME: 14:00
LOCAL: ON-LINE
TITLE:

REPRODUCTIVE EVALUATION OF BUFFALO FEMALES SUBJECTED TO FTAI AND MONOMORPHIC FOR THE ANTI-MULLERIAN HORMONE (AMH) GENE, IN THE AMAZON.

KEY WORDS:

AMH; Buffaloes; Fetility; Polymorphism.

PAGES: 47
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SUMMARY:

 Reproductive management in buffaloes is essential for the productivity of the Brazilian agricultural sector, especially in the Amazon region. The anti-mullerian hormone (AMH) is considered a significant marker of ovarian reserve, and understanding the relationship between genotypes and fertility is important for livestock farming. Thus, the present study aimed to investigate the presence of polymorphisms in exon 5 of the AMH gene and their associations with reproductive efficiency in females. The research was conducted with 54 multiparous Murrah buffaloes located on a property in the municipality of Bujaru, Pará, under controlled feeding and watering conditions. The females underwent a fixed-time artificial insemination (FTAI) protocol. Blood samples for DNA extraction and ultrasonographic evaluation of the ovaries were collected on day 11 of the protocol. DNA extraction and amplification of the AMH gene were performed using PCR techniques, and the purification of the products was carried out for sequencing. Statistical analyses were conducted using SAS software, considering a significance level of 0.05, and ANOVA methods were employed to compare the means of the reproductive variables. The results showed the absence of polymorphisms in exon 5 of the AMH gene in buffaloes and a significant difference in the count of antral follicles (AFP) between pregnant and non-pregnant buffaloes (p = 0.02). However, variables related to ovarian volume (V) and dominant follicle (DF) did not show significant differences, indicating that these factors may have a less evident role in reproductive status. The findings suggest the conservation of the AMH gene and the importance of antral follicles as predictors of reproductive efficiency in buffaloes. The research highlights the need for future studies in other regions of the AMH gene and the association with genetic factors that may influence fertility. Additionally, the results may contribute to the future development of more effective assisted reproduction strategies aimed at increasing productive efficiency in buffalo herds in the Amazon region.

 

COMMITTEE MEMBERS:
Presidente - 1556914 - EDNALDO DA SILVA FILHO
Externa à Instituição - ELIZABETH MACHADO BARBOSA - UNIFAP
Interna - ***.347.322-** - NATHALIA NOGUEIRA DA COSTA - UFPA
Externa à Instituição - SIMONE DO SOCORRO DAMASCENO SANTOS