Banca de QUALIFICAÇÃO: RAMON BARROS DA CUNHA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : RAMON BARROS DA CUNHA
DATE: 17/06/2022
TIME: 10:30
LOCAL: Setor de reprodução animal/ISPA/UFRA
TITLE:

INFLUENCE OF IRRIGATION AND PREOVULATORY FOLLICLE DIAMETER ON PREGNANCY RATE IN DAIRY BUFFALOES

KEY WORDS:

Keywords: Doppler ultrasound, pregnancy, buffaloes

PAGES: 41
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

This study aimed to evaluate the influence of irrigation and preovulatory follicle diameter on pregnancy rate  in dairy buffaloes

 submitted to fixed-time artificial insemination. 151 multiparous dairy buffaloes with more than 30 days calving aged between 4 and 8 years were used. 

The D0 synchronization protocol, the buffaloes received an intravaginal device containing 1g of P4 (Primer®, Agener União Química),

 application of 2.0mg of BE (Fertilcare Sincronização®, MSD Saúde Animal) at 16:00. On D9, also at 16:00, the device was removed, 0.265 mg 

of closprostenol sodium (Ciosin®, MSD Saúde Animal) and 300 IU of eCG (Folligon®, Intervet International) via i.m. 

On D11 at 4 pm, 0.1 mg of GnRH (Fertagyl®, Intervet International) was administered i.m. On D12, 16 hours after 

the application of GnRH (at 8:00 am) at the time of insemination, the animals were evaluated for follicle diameter and 

 its degree of irrigation, by transrectal ultrasound using a two-dimensional Doppler ultrasound (Chison Eco Vet, Beijing, China). ) 

equipped with a multi-frequency microconvex transducer, in which two-dimensional and Color-Doppler evaluation were performed, 

and the images were later evaluated through a software, the blood flow and associated tissue to calculate the vascularization index. 

The overall pregnancy rate was 32.45% (49/151). Smaller area follicles (99.01±16.18amm) were correlated with pregnant females, 

while in non-pregnant females the area of follicles was greater (117±46.17bmm) (P<0.05). The mean and standard deviation

 of the other variables studied are shown to be smaller in pregnant buffaloes, in relation to the means and standard deviation in non-pregnant buffaloes. 

However, we highlight the percentage of vascular perfusion (visual vascularization) for pregnant (19.04±5.2%a) and non-pregnant (56.57±9.95%b) buffaloes (P<0.05), 

the other variables, such as Fol/width distance ( mm), Fol/height distance (mm), Fol perimeter (mm) and wall area + Fol (mm) we did not obtain statistical difference 

(P>0.05). We can conclude that the color Doppler is the indicator instrument, of great precision to the deficiency of vascularization 

of the pre-ovulatory follicle, as well as, by the area of the largest follicles, which not always the largest area, is related to predicting fertilization.

BANKING MEMBERS:
Presidente - 388431 - HAROLDO FRANCISCO LOBATO RIBEIRO
Interno - 1556914 - EDNALDO DA SILVA FILHO
Interno - 2495071 - SEBASTIAO TAVARES ROLIM FILHO