Polymorphism in the HSP70 gene associated with reproduction in dairy cows
Fertility, milk, PCR
Aiming to reduce the possible impacts of heat stress on the production of dairy cattle, genetic programs have been used for the selection of heat resistant animals. Several studies have been associating the Hsp70 gene with phenotypic characteristics of economic interest, associating resistance to mastitis, weaning weight of calves, pregnancy rate in crossbred B. taurus and B. indicus cows. Thus, the objectives of the present study will be to identify single nucleotide polymorphisms (SNP) located in the promoter region of the Hsp70 gene and to evaluate the associations between Hsp70 SNPs and the reproductive efficiency of dairy cows. Evaluating phenotypic characteristics such as pregnancy rate up to 150 days after Artificial Insemination, conception rate. Reproductive data of 200 crossbred dairy cows raised in the northeast region of Pará, subjected to management with high temperature and relative humidity will be evaluated and will be divided into high and low fertility animals. These data will be associated with polymorphisms found in the Hsp70 gene and verified if there is any relationship with the reproductive characteristics evaluated. The data will be tabulated in electronic spreadsheets and submitted to the statistical program SAS Ondemand (2021) and the statistical test ANOVA and Pearson Correlation Test, with 5% significance.