MICROPARASITES IN GONADS OF TWO SPECIES OF AMAZONIAN FISH: HISTOPATHOLOGICAL ANALYSIS AND IN VITRO TESTS WITH ROSEMARY ESSENTIAL OIL.
Keywords: Microparasites; rosemary essential oil; histopathology; Amazonian fish.
This study investigates the occurrence of microparasites in the gonads of two species of fish of ecological and economic importance in the Amazon basin: Pygocentrus nattereri (red piranha) and Colossoma macropomum (tambaqui). The research is divided into two main fronts. The first consists of the histopathological analysis of the gonadal tissues of these fish, aiming to identify the presence and morphological effects of parasitic infection. The second proposes the development of in vitro tests to evaluate the efficacy of rosemary essential oil (Rosmarinus officinalis) as an antiparasitic agent against Myxozoan spores. The use of phytotherapeutics with antimicrobial and antiparasitic action represents a promising and sustainable approach for the control of infections in aquatic organisms, especially in native species relevant to human food. To date, in vitro tests have not yet been initiated, but the essential oil has already been purchased and is available for the experimental phase. Histopathological analysis performed on samples of P. nattereri revealed the presence of cysts containing spores of Myxobolus sp. (Myxozoa: Myxobolidae) located in the ovarian parenchyma, associated with oocyte degeneration. These findings indicate a possible impairment of reproductive function, reinforcing the need for parasite control strategies in fish from the Amazon region.