Banca de DEFESA: ROBERTA DE ARAÚJO SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ROBERTA DE ARAÚJO SILVA
DATE: 28/01/2022
TIME: 14:00
LOCAL: Auditório do ISPA-UFRA
TITLE:

Genetic Characterization of the Promoter Region of the FSH Receptor Gene in Quarter Horse Mares

KEY WORDS:

Polymorphisms, equine, SNP, embryo, FSHR.

PAGES: 35
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Inseminação Artificial Animal
SUMMARY:

The Quarter Horse is a breed that stands out for its strength, explosion and versatility, thus being used in various equestrian sports, where the drum and vaquejada competition are highlighted. Therefore, assisted reproduction is challenging both in terms of sperm and follicular quality for artificial insemination and/or in vitro fertilization. The characterization of genes involved in the production of gametes such as the follicle stimulating hormone receptor (FSHR) serves as a candidate in the application of genetic improvement programs in the species. Therefore, the objective of this work was to characterize part of the promoter region and the 5'untranslated region (5ÙTR) of the FSHR gene in quarter-mile horses and to associate it with the production of embryos produced in the 2021 season. , peripheral blood was collected from 81 mares from three farms for DNA extraction, followed by amplification of 707 bp of the promoter region of the FSHR gene. Then the PCR products were purified and sequenced by the Sanger method. The sequences were aligned and compared between mares. Allelic and genotypic frequencies and inbreeding coefficients were determined by the GENEPOP program. The analyzes of possible sites for the binding factors were determined by the ALIBABA2.0 program. The association of haplotypes with the number of embryos was performed using the PROC MIXED of SAS onDemand with a significance level of 0.05. Three polymorphisms (SNPs) were identified, two in the promoter region (-217G>T and -195G>A). In SNP-217, the most frequent allele and genotype are G=0.939 and GG=0.902. In SNP -195, the most frequent allele and genotype are G=0.896 and 0.841. One SNP was identified in 5-ÙTR at position 127A>G, with the most frequent allele and genotype being G=0.729 and GG=0.602. Linkage disequilibrium was significant between SNPs -217 and -195. 74 segments with potential binding sites were identified in the wild-type sequence, being at position -217 the Sp1 and TFAP-2alpha and at position 195 the site for GR, while in the mutant sequence 71 were identified as the aforementioned missing binding sites. A total of 12 haplotypes were identified, 4 with a frequency greater than 0.05 and the haplotypes had no significant association (P>0.05) with the number of embryos produced in the 2021 season. The promoter region of the FSHR gene demonstrated be polymorphic in Quarter Horse horses and there was no association with the number of embryos, but it is promising as a genetic marker for other traits associated with reproduction in horses.

BANKING MEMBERS:
Presidente - 1556914 - EDNALDO DA SILVA FILHO
Interno - 515.616.542-72 - MOYSÉS DOS SANTOS MIRANDA - UFPA
Externo ao Programa - 1557097 - IGOR GUERREIRO HAMOY
Externa à Instituição - ELIZABETH MACHADO BARBOSA - UNIFAP