Banca de QUALIFICAÇÃO: JULIANA VASCONCELOS FIGUEIREDO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JULIANA VASCONCELOS FIGUEIREDO
DATE: 30/04/2024
TIME: 15:00
LOCAL: Google meet
TITLE:

VARIABILITY OF THE ANTI-MULLERIAN GENE (AMH) ASSOCIATED WITH FOLLICULAR CHARACTERISTICS AND FERTILITY IN BUFFALOES

KEY WORDS:

AMH; Bubalus bubalis; Exon 5; Fertility; Molecular Marker.

PAGES: 35
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SUMMARY:

The buffalo herd, estimated at a global population of 1.5 million, has played a significant role in national livestock farming, with the North region being the main producer, contributing to both milk and meat production. To improve breeding and production efficiency, genetic factors must be considered. The growth of the global market and technological advances have led to new demands for better quality products and greater food safety. Genetic and environmental factors can affect the characteristics of animals, such as selection into specific environments and behavior. Molecular markers such as anti-Mullerian hormone (AMH) can help determine the amount of healthy antral tissue in the ovaries. AMH regulates follicular growth and prevents premature follicular loss, while polymorphisms in the gene can cause genetic variations that interfere with the buffalo's genetic factors. Therefore, the present work aims to detect variations in the AMH gene in the exon 5 region in association with ovarian characteristics in predicting fertility in buffaloes. For this, blood samples were collected from 25 buffaloes and ultrasonographic detection of both ovaries on D10 of an IATF protocol to measure the diameter of the FD and associate the number of follicles of females belonging to a property located in the municipality of Bujaru, Pará. Next, DNA extractions were carried out using the phenolchloroform method from all samples. A temperature gradient test was performed for the AMH gene, which was standardized at 61.2°C. Subsequently, conventional PCRs were performed on 25 samples. Therefore, based on this information, analyzes will be carried out on the remaining animals to continue detecting the AMH SNP, as well as gene expression profile analysis and association of these data with reproductive rates in buffaloes.

COMMITTEE MEMBERS:
Presidente - 1556914 - EDNALDO DA SILVA FILHO
Interna - ***.347.322-** - NATHALIA NOGUEIRA DA COSTA - UFPA
Interno - 2495071 - SEBASTIAO TAVARES ROLIM FILHO
Externa à Instituição - ELIZABETH MACHADO BARBOSA - UNIFAP
Externa à Instituição - SIMONE DO SOCORRO DAMASCENO SANTOS - UFPA