Banca de DEFESA: JAMYLE CAROLLYNE MELEM SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JAMYLE CAROLLYNE MELEM SANTOS
DATE: 29/08/2022
TIME: 14:00
LOCAL: Videcoconferência via zoom
TITLE:

BIOCHEMICAL BIOMARKERS OF BUFFALO CALVES FROM BIRTH TO 18 MONTHS OF AGE

KEY WORDS:

Buffalo, metabolic profile, age factors.

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Fisiopatologia da Reprodução Animal
SUMMARY:

The hematological studies of the buffalo species available in the literature do not yet allow establishing all the standards and normality values for the species, thus, this work aims to determine the biochemical profile of buffalo calves from birth to weaning considering the influence of sex and age on the parameters obtained. The study will be carried out at the Bioterium Bubaline Dairy Unit Eva Daher Abufaiad/Ufra, Belém campus, Pará. 24 neonate buffalo animals will be used, crossbred Murrah x Mediterranean, 12 males and 12 females. All calves will remain with their dams throughout the experimental phase. Each calf will be clinically examined before sampling to estimate health status, and those without clinical changes at birth will be eligible for inclusion in the study. Inclusion criteria for calves will be determined by alert physical examination and vigor, normal rectal temperature (> 38 and < 39.5°C) and normal vital parameters (heart and respiratory rates, mucosal color and capillary refill time) at birth . There will be 22 harvests of each animal up to 18 months of age. On each day of collection, 10 mL of blood samples will be collected by puncture of the external jugular vein, using disposable needles (25 x 8mm) coupled to vacuum collection tubes left at room temperature for clot retraction and subsequent centrifugation at 3,000 g for 15 minutes, with 2.0 mL aliquots of blood serum being separated and stored in Eppendorf-type tubes, previously identified and kept at a temperature of -20ºC, until the moment of biochemical analysis. Biochemical determinations will be performed using commercial kits using the automated biochemical analyzer. In the blood serum will be determined the analysis of urea, creatinine, alkaline phosphatase, total proteins, globulin, albumin, albumin/globulin ratio, glutamic oxaloacetic transaminase and Gamma Glutamyl Transferase.

BANKING MEMBERS:
Presidente - 1368855 - RINALDO BATISTA VIANA
Externo à Instituição - DAMAZIO CAMPOS DE SOUZA
Externo à Instituição - JOSE DANTAS RIBEIRO FILHO - UFV
Externa à Instituição - WALESKA DE MELO FERREIRA DANTAS