Banca de DEFESA: ANDREY DE SOUSA MIRANDA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANDREY DE SOUSA MIRANDA
DATE: 18/07/2022
TIME: 14:00
LOCAL: Sala virtual
TITLE:

SUPPLEMENTATION WITH FAT PROTECTED DURING MID-GESTATION IN EWES ON GENE EXPRESSION, HISTOLOGICAL PROFILE AND MEAT QUALITY OF THE PROGENY

KEY WORDS:

Adipogenesis. Gluconeogenesis. Myogenesis. Maternal nutrition. Fetal programming. Histological profile. Meat quality.

PAGES: 63
BIG AREA: Ciências Agrárias
AREA: Zootecnia
SUBÁREA: Produção Animal
SPECIALTY: Criação de Animais
SUMMARY:

Maternal nutrition provided by the availability of nutrients in the diet during pregnancy can directly affect the progeny on performance characteristics and meat quality, a mechanism known as fetal programming. Based on this, the objective was to evaluate the effects of calcium salt supplementation of soybean oil in the maternal diet of crossbred Dorper x Santa Inês ewes during the second half of gestation on maternal and offspring gene expression; as well as the meat quality and histological profile of the offspring. At 75 ± days of gestation, 43 multiparous ewes (44.10 ± 6.87 kg BW, 2 ± 1 parturition) hairless (Dorper × Santa Inês crossbred) pregnant with females (n = 18) and males (n = 25 ), were allocated to individual pens and randomly assigned to the following treatments: (1) Control (CON; n = 21) – supply of a basal diet formulated to meet the basic nutritional requirements of pregnant ewes with a single lamb, or (2) Supplementation ( CSSO; n = 22) – basal diet plus supplementation with calcium salts from soybean oil (rumen-protected fat) receiving daily 1.5g/kg of body weight (5% based on DM. Liver and adipose tissue of ewes at 130 days of gestation, and adipose and muscle tissue of the progeny at 15 days of age and at slaughter for the evaluation of myogenic, fibrogenic, adipogenic, and gluconeogenic markers. At slaughter, samples of the Longissimus lumborum muscle were also collected for determination from the hi profile histological and qualitative aspects of meat. An interaction effect between maternal nutrition and offspring sex was observed, where CSSO ewes pregnant with females showed higher expression of markers related to adipogenesis and lipogenesis PPARy (P = 0.01) in adipose tissue and ACACA (P < 0.001) in liver tissue . There was interaction (P < 0.05) for area, perimeter and diameter of the progeny muscle fibers, with favorable responses observed for CSSO females compared to the other treatments. There was a trend of sexual dimorphism on the histological profile of subcutaneous adipose tissue, in which females showed ~24% and 12% higher adipocyte area and perimeter (P < 0.10) than males, respectively. However, the fat cell population tended to be ~14% lower (P = 0.08) in females. There was an interaction effect on the relative expression of ZFP423 (P < 0.001) in female progenies from CSSO mothers at birth and PPARy (P < 0.001) for samples at slaughter. Furthermore, the myogenesis of CSSO progenies was affected by having higher mRNA expression for MyOG (P = 0.05). Progenies from CSSO mothers showed lower expression of the type I fiber marker (MyHC I) and higher PPARy (P = 0.01). CSSO progenies showed greater subcutaneous fat thickness (EGS, P = 0.04), maximum fat thickness over Longissimus lumborum (measured GR; P = 0.05) and total losses (cooking and thawing; P = 0.04) that CON Consistent with these responses, higher mean values of visceral fat deposition and carcass coverage (P < 0.001) were observed in females, in addition to higher EGS and carcass GR measure (P = 0.02). Therefore, maternal nutrition with calcium salts from soybean oil during the second half of pregnancy alters fetal growth and trajectory in a sex-dependent manner, due to the modification in the potential for postnatal development of skeletal muscle and subcutaneous adipose tissue.

BANKING MEMBERS:
Externo à Instituição - GERMÁN DARÍO RAMÍREZ-ZAMUDIO
Externo à Instituição - TATHYANE RAMALHO SANTOS GIONBELLI
Presidente - 1354679 - KALIANDRA SOUZA ALVES
Externa à Instituição - KAROLINA BATISTA NASCIMENTO - UFLA
Interno - 1894011 - RAFAEL MEZZOMO