INOCULATION OF NITROGEN FIXING BACTERIA VIA SEED AND LEAF IN Megathyrsus maximus cv. ZURI
Azospirillum brasilense; Methylobacterium sybioticum; plant growth promoting bacteria
The objective of this study was to evaluate the effects of seed and foliar inoculation with Azospirillum brasilense and Methylobacterium symbioticum on the production and nutritional parameters of Megathyrsus maximus cultivar BRS Zuri. Six treatments were applied as described below: negative control - 0 kg N (T1), positive control - 100 kg N (T2), seed inoculation with A. brasilense (T3), seed inoculation with M. symbioticum (T4), seed inoculation with A. brasilense and co-inoculation via foliar application of M. symbioticum (T5), and foliar inoculation with M. symbioticum (T6). A completely randomized design with five replications was adopted. The experimental units consisted of 10 dm³ pots with a sand substrate, balanced fertilization, and five plants per pot after thinning. Evaluations included total forage mass, root mass, shoot/root ratio, height, chlorophyll content, number of tillers, dry matter, mineral matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber. Inoculation with nitrogen-fixing bacteria (NFB), regardless of the inoculation method or NFB type, increased root dry mass, number of tillers, and neutral detergent fiber. The negative control showed lower means compared to the other treatments for the variables total forage dry mass, height, chlorophyll, and crude protein. Inoculation and foliar co-inoculation with M. symbioticum promoted higher means compared to the negative and positive controls for tillers and acid detergent fiber. It is concluded that inoculation with NFB improved the development of Zuri grass, comparable to nitrogen fertilization, with positive effects on root growth and tillering (M. symbioticum). The dose of 100 kg N/ha partially inhibited biological fixation. It is recommended to combine bacterial strains and adjust N doses to optimize sustainability and productivity.