Banca de QUALIFICAÇÃO: ANDREY DE SOUSA MIRANDA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ANDREY DE SOUSA MIRANDA
DATE: 27/05/2022
TIME: 14:00
LOCAL: Sala virtual- Google meet
TITLE:

Soybean oil calcium salt supplementation during the second half of pregnancy in Dorper x Santa Inês ewes: impact on maternal hepatic gene expression, histological profile and offspring meat quality

KEY WORDS:

Histological analysis. Gene expression. Maternal nutrition. Fetal programming. Meat quality.

PAGES: 40
BIG AREA: Ciências Agrárias
AREA: Zootecnia
SUBÁREA: Produção Animal
SPECIALTY: Criação de Animais
SUMMARY:

The manipulation of maternal nutrition during pregnancy can have direct effects on its impact on the offspring through fetal programming. Thus, the objective was to evaluate the effects of supplementation with Ca salts of soybean oil during the second half of gestation in Dorper x Santa Inês ewes on maternal hepatic gene expression; characteristics of fat deposition, gene expression, meat quality, histological profile of the progeny. The experiment was carried out in the Small Ruminant Confinement Sector of the Federal Rural University of Amazonia - UFRA/Campus Parauapebas, 60 multiparous multiparous sheep (Dorper and Santa Inês crossbreds) were used with synchronized IOC. The experimental treatments were distributed to pregnant ewes in the second half of gestation until parturition, as follows: 1) Control (CON) - Diet formulated to meet the basic nutritional requirements for pregnant ewes with a single lamb, 2) Supplementation with calcium salts Soybean Oil (SUP) – Basal diet added Supplementation (1.5% body weight/5% based on DM) in the form of rumen-protected fat. Biopsies of the ewes' liver and adipose tissue were performed at 130 days of gestation; and biopsy of adipose tissue and muscle at 15 days after birth and at slaughter of the progeny. Fat and muscle tissue samples were collected at slaughter for histological analysis. After slaughter, samples of the Longissimus lumborum muscle were collected for quality analysis. There was an interaction effect (P<0.05) between maternal food treatment and progeny sex type for the histomorphometric parameters of skeletal muscle tissue, with the exception of the number of muscle fibers. Progenies from SUP matrices showed greater area, perimeter and diameter of the longissimus muscle fiber (P<0.05) compared to progenies from matrices from the control group, and consequently a tendency towards a lower (P=0.07) number of muscle fiber/100 thousand µm². The type of sex had an effect (P>0.05) for all fat deposit variables evaluated, where the highest mean values were observed in offspring of females, thus presenting greater deposition of visceral fat and fat covering the carcass. Progenies from SUP ewes showed greater subcutaneous fat thickness (1.67 mm), maximum fat thickness over the Longissimus lumborum (5.47 mm) and total losses (29.07 %). There was an effect of sex type (P<0.05) on fat deposition, where offspring of females had higher EGS (1.82 mm), GR (3.01 mm) of the carcass and red content a* (19.71), in contrast to offspring of males showed higher final meat pH (5.44). Therefore, the manipulation of the maternal diet from the second half of pregnancy with calcium salts of soybean oil aiming at improving performance is advantageous, because during the fetal phase there is a predominance of multipotent cells capable of differentiating into muscle and adipose tissue, which will mainly influence the fat deposition and meat quality of female offspring.

BANKING MEMBERS:
Presidente - 1354679 - KALIANDRA SOUZA ALVES
Interno - 1894011 - RAFAEL MEZZOMO
Externa à Instituição - IRAIDES FERREIRA FURUSHO GARCIA - UFLA
Externa à Instituição - KAROLINA BATISTA NASCIMENTO - UFLA