Association of Testicular Morphometry with Spermal Gene Expression in Bufalloes ( Bubalus bubalis)
Morphometry, Gene Expression, PCR-RT
ABSTRACT
The buffalo livestock is responsible for moving the economy in families in the northern
region. In order to exploit the economic, productive and reproductive potential of a species,
basic knowledge of its physiology is required. From this point of view, information about
buffaloes is incipient, especially regarding the gene expression, physiology and pathology of
the male. The expansion and higher productivity of the buffalo herd will only exist if the
genetic knowledge of the relevant traits is associated with reproductive efficiency, thus
selecting the most suitable for reproduction and having superior genetics for reproductive
traits. RNA analysis can provide important information on gene expression. Thus, the
objective of this study was to evaluate the testicles obtained from the slaughterhouse, relating
the anatomopathological, morphometric findings of the seminiferous tubules with the
identification of P450scc, 3B-HSD, P450c17, CYP19A1, STAR, PTGS1 and PTGS2 genes,
linked to spermatogenesis. in the buffalo testicle samples in the states of Amapá and Pará