Cortisol, estrogen and progesterone fecal metabolite profiles in Sauim-una females (Saguinus ursulus, Primate, Callitrichidae)
progesterone, estrogen, cortisol, ovarian cycle, Saguinus ursulus.
Sauim-una (Saguinus ursulus) is an Amazonian primate that has not yet evaluated its conservation status, but has been under severe pressure due to habitat destruction and fragmentation. Unfortunately, these animals have no reproductive success in captivity and little is known about their reproductive biology. The ability to determine hormonal profiles of primate populations using non-invasive techniques can assist in reproductive management, allowing stress analysis due to physiological responses related to environmental changes. The objective of this study was to evaluate the reproductive function of sauim-una females by fecal metabolic profile analysis, validating the method for species, by means of progesterone (P), estrogen (E) and cortisol (C) hormonal analyzes. enzyme immunoassay (EIA). The assay was validated by the precision (pc) and parallelism test, showing that the dilutions (P = 1: 5001, pc = 96%; E = 1: 128, pc = 107%; C = 1: 32, pc = 116%) were parallel to the EIA reference curves. The values of R² were 0.88; 0.96 and 0.96 for P, E and C metabolites respectively, in all cases p <0.005. The high correlation observed between the sample pool curves and the diagnostic sets for the dosages performed suggests that the metabolites of the extracts interacted with the antibody similar to the standard hormone. Monitoring of hormone levels can be applied to wild life populations and is of fundamental importance for captive reproductive management.