Banca de DEFESA: SUELLEN DA GAMA BARBOSA MONGER

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : SUELLEN DA GAMA BARBOSA MONGER
DATA : 30/08/2019
HORA: 14:00
LOCAL: Sala 1 da Zootecnia básica
TÍTULO:

Molecular identification and antimicrobial resistance of pathogenic Escherichia coli and Salmonella sp. isolated from pigs raised in the State of Pará

PALAVRAS-CHAVES:

Enteropathogens, swine, antimicrobial resistance, State of Pará

PÁGINAS: 66
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
ESPECIALIDADE: Epidemiologia Animal
RESUMO:

Swine is the main source of animal protein in the world and Brazil stands out as a world producer and exporter of pork products. However, in the northern region, pig farming is characterized as a complementary source of income, and with little attention to hygienic-sanitary care, which favors those of enteric bacterial infections that have a major impact on the pig industry worldwide, including Some agents pose a risk to public health. One of the most widely adopted strategies to prevent and control enteric infections has been antimicrobial therapy. However, the lack of criteria for the proper and safe use of antimicrobials may result in selection of resistant bacteria. In the Amazonian ecosystem, little is known about the occurrence of enterobacteria in slaughter pigs. Thus, the objective was to investigate the occurrence of pathogenic Escherichia coli and Salmonella sp. in pigs raised in the State of Pará, in addition to verifying the antimicrobial resistance profile of pathogenic strains, and thus providing data that can be used to better direct control measures. 200 swine fecal samples were collected from swine from farms located in the State of Pará and processed at the Enterobacterial Laboratory of Instituto Evandro Chagas, Ananindeua, PA. For isolation of E. coli, samples were sown in Escherichia coli broth and Gram negative (GN) broth, incubated and then seeded on MacConkey Agar. Suspected colonies were identified in TSI (Triplice Sugar Sugar) for further biochemical characterization. The characterization of the pathotype was performed from amplification by PCR (Polymerase Chain Reaction) Multiplex technique. For isolation of Salmonella sp., Samples were inoculated into the Selenite Cystine and Rappaport enrichment broths and, after incubation, were seeded in the selective culture media SS indicators and on Xylose-Lysine-Descarboxylate (XLD) agar. Suspicious colonies were identified on TSI for later biochemical identification and confirmed by PCR. The susceptibility to antimicrobial agents of the isolates was evaluated using the automated VITEK® 2 Compact system (bioMérieux). Data were tabulated and evaluated using statistical software Statistical Analysis System (SAS, 2001), using the chi-square statistical test (χ2) with a significance level of 5%. To determine the risk factors, the results of the records were tabulated and the odds ratios test used for each investigated variable, calculated considering as dependent variable the animal status (positive or negative) for the pathogenic bacterium. Of the 200 animals, 39.5% (79/200) presented pathogenic isolates. Of these, 22% of the animals with enteropathogenic (44/200) E. coli, 11% (22/200) enterohemorrhagic E. coli, and 10.5% (21/200) enteroinvasive E. coli were diagnosed by PCR. 3% (6/200) with Salmonella sp. Resistant samples were found for Nalidixic Acid, Sulfamethoxazole, Ampicillin, Amikacin, Gentamycin, Ceftriaxone, Cephalothin, Beta-lactams.

MEMBROS DA BANCA:
Presidente - 388501 - WASHINGTON LUIZ ASSUNCAO PEREIRA
Externo ao Programa - 1368855 - RINALDO BATISTA VIANA
Externo ao Programa - 2150577 - ALEXANDRE DO ROSARIO CASSEB
Externo ao Programa - 2494981 - ADRIANA MACIEL DE CASTRO CARDOSO JAQUES
Externo à Instituição - SANDRO PATROCA DA SILVA - NENHUMA
Externo à Instituição - HILMA LÚCIA TAVARES DIAS - UFPA