Banca de QUALIFICAÇÃO: CARLOS CHRISTIAN SANTOS DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : CARLOS CHRISTIAN SANTOS DA SILVA
DATE: 27/02/2025
TIME: 14:00
LOCAL: on-line pelo Google Meet
TITLE:

DETECTION AND MOLECULAR CHARACTERIZATION OF HEPATITIS E VIRUS (HEV) IN BATS IN THE STATE OF PARÁ, BRAZIL

KEY WORDS:

Wild animals. Viruses, Health. Amazon.

PAGES: 29
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

Hepatitis E is a viral disease that affects millions of people worldwide and is a public health concern. It has a tropism for liver tissue and triggers acute liver changes, not leading to death, but with persistent hepatitis, and is considered a global public health problem. The search for new hosts provides important data that can be used to better understand the evolution and its relationship with humans, geographic distribution, genetic variability, prevention and the possibility of these animals being a reservoir of potential contamination in humans, since the crossing of interspecies barriers has been reported. Given this scenario, the objective of this study was to investigate the occurrence of the Hepatovirus E genus in bats in the State of Pará, Brazil. The capture will be carried out at the Universidade Federal Rural da Amazônia, Campos Belém do Pará and Castanhal/PA, as well as in some other strategic regions in the metropolitan region of Belém, both captive and free-living. After capture, euthanasia will be performed by exsanguination, through intracardiac puncture or eustasis by pharmaceutical overdose. Molecular analysis will initially be performed on 200 samples of liver and some viscera from bats, through the extraction of total RNA and DNA. PCR assays will be developed for partial amplification of genes from conserved regions for HEV. The amplicons from positive samples will be purified and sequenced in forward and reverse directions; consensus sequences will be obtained using BioEdit software (v7.2.6) and submitted to the BLAST platform. They will then be aligned with a database containing representative sequences for HEV genotypes and subspecies available in the Genbank database. For phylogenetic analysis, the Maximum-Likelihood method and the Kimura 2-parameter model will be used in MEGA software v7.0 for taxonomic identification of isolates. Nucleotide and amino acid distances will be calculated using Geneious software v8.1.3.

COMMITTEE MEMBERS:
Presidente - 388501 - WASHINGTON LUIZ ASSUNCAO PEREIRA
Externa ao Programa - 2494981 - ADRIANA MACIEL DE CASTRO CARDOSO JAQUES - UFRAExterna à Instituição - HILMA LÚCIA TAVARES DIAS - UFPA
Externo à Instituição - PEDRO EDUARDO BONFIM FREITAS - IEC
Externo à Instituição - SANDRO PATROCA DA SILVA - NENHUMA