Fetal bone development in the black agouti (Dasyprocta fuliginosa), determined using ultrasonography
Hystricomorphic rodent, skeletal system, fetal growth.
This study describes bone development during the fetal phase of the black agouti (Dasyprocta fuliginosa), comparing it with other precocial or altricial species, and its relationship with the species’ adaptive strategies. 33 embryos/fetuses obtained over the course of 17 years through collaboration with local subsistence hunters in the Amazon were analyzed. Measurements of mineralization of the axial and appendicular skeletons were performed by ultrasonography using a 10–18-MHz linear transducer. The chronological order of occurrence of mineralization in relation to the total dorsal length (TDL) was skull, ribs, vertebral bodies (TDL = 8.2 cm); clavicle, scapula, humerus, radius, ulna, ilium, ischium, femur, tibia, and fibula (TDL = 8.2 cm); metacarpi/metatarsi and pubis (TDL = 9 cm); phalanges and tarsus (TDL = 13.2 cm). Carpus and patella were not observed. Secondary ossification centers first appeared in the distal epiphysis of the radius and femur, and in the proximal and distal epiphysis of the tibia (TDL = 13.2 cm) and tibial proximal epiphysis (TDL = 18.4 cm). Advanced fetuses (TDL > 21.5 cm, 93.5% gestational period) presented mineralization in all primary and most secondary centers. Compared to other species, black agouti neonates have a well-developed skeletal system at birth, which is important for their independent postnatal locomotion. Our results may contribute to the monitoring of bone development in other wild species, helping us to understand their life history, and serving as parameters for comparisons between precocial and altricial mammals.