Propylene glycol in the feeding of santa ines lamb in feedlot
1,2 propanediol; alternative foods; PEPCKc and PPARG genes; ruminant nutrition.
During the manufacture of biodiesel propylene glycol (PG) is obtained, which has gluconeogenic properties and can be used as an energy source in diets for ruminants. When PG is given orally or by ruminal infusion, it can be absorbed by the ruminal papillae, fermented, or continued in the gastrointestinal tract. When direct absorption in the rumen epithelium occurs, it is metabolized in the liver and will contribute to the synthesis of glucose, when fermentation occurs, it is used as a substrate for the production of propionate, that is, PG can be considered as a direct or indirect precursor of glucose in ruminants. Thus, the aim will be to evaluate the effect of propylene glycol on productive performance, physiological parameters, carcass and meat characteristics and expression of genes that regulate gluconeogenesis, lipid content and marbling index of Santa Inês lamb meat. The project will have two phases, in the first twenty lambs will be distributed in a completely randomized design in four treatments, defined by the absence (control) and levels of 2%, 4% and 6% of PG in the water supplied to the animals. The animals will be fed for 27 days, with 21 days of adaptation and 6 days of collection to evaluate the intake and apparent digestibility of dry matter, crude protein and neutral detergent fiber, nitrogen balance and blood parameters. The results will define the inclusion of propylene glycol for the second phase, where the same animals will be used distributed in two treatments defined by the absence and inclusion of PG, where they will be fed for 45 days to evaluate the consumption and apparent digestibility of dry matter, crude protein and neutral detergent fiber, nitrogen balance and blood and rumen parameters, performance, confinement time and biometric measurements, will subsequently be slaughtered to assess carcass and meat characteristics, collection of epithelial tissue from the gastrointestinal tract for histological evaluation, from liver tissue to analyze the expression of genes that regulate gluconeogenesis and Longissimus lumborum muscle to analyze the expression of genes that regulate lipid contente.