Banca de QUALIFICAÇÃO: CINTIA LUANA PINHEIRO SANTOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : CINTIA LUANA PINHEIRO SANTOS
DATE: 22/10/2021
TIME: 14:00
LOCAL: Google Meeting
TITLE:

ASSOCIATION OF THE g4467 G>A POLYMORPHISM IN THE 3´ UTR OF THE INTERFERON GAMA (IFNG) GENE WITH THE GENE EXPRESSION PROFILE OF THE IFNG AND bta-miR-125b microRNA IN BUFFALOES WITH OR WITHOUT HEMOPARASITOSES.

KEY WORDS:

Buffaloes. Hemoparasitosis. IFNG. Molecular Marker. SNP.

PAGES: 30
BIG AREA: Ciências Agrárias
AREA: Zootecnia
SUBÁREA: Genética e Melhoramento dos Animais Domésticos
SUMMARY:

The aim of the present study was to associate the g4467 G>A polymorphism present in the 3' UTR region of the Interferon gamma gene (IFNG) with the gene expression profile of the IFNG gene together with a miRNA, the bta-miR-125b, in buffaloes without hemoparasitosis. Therefore, blood samples were collected from 191 buffaloes. Then, molecular biology laboratory procedures were performed. DNA extraction from whole blood was performed using the phenolic method, then PCR and RT-PCR techniques were used for the diagnosis of hemoparasites, among which were: Babesia spp. Trypanosoma spp. and Anaplasma marginale. Subsequently, the PCR-RFLP technique was performed to genotype the polymorphism (g4467 G>A) in the IFNG gene in 191 buffaloes. From the genotypes generated, the analyzes of genetic diversity such as allelic and genotypic frequencies, Hardy Weinberg equilibrium and inbreeding coefficient were assessed by the GENEPOP program. The results of the PCRs techniques used for the diagnosis of hemoparasitosis were all negative in the 191 female buffaloes for the three agents. The PCR-RFLP technique performed in 63 buffaloes revealed different band patterns for the studied polymorphism which were the GG and AA genotypes, in the proportions 3.17% and 96.83%, respectively, the A allele being the most frequent (0 .9683) in the studied population. The polymorphism presented deviation in the Hardy-Weinberg equilibrium (EHW) (P<0.05) and heterozygous deficit, characterized by a positive FIS value (1,0161).

BANKING MEMBERS:
Presidente - 1556914 - EDNALDO DA SILVA FILHO
Externo ao Programa - 2150577 - ALEXANDRE DO ROSARIO CASSEB
Externo ao Programa - 1557097 - IGOR GUERREIRO HAMOY
Externa ao Programa - 2116073 - PRISCILA DI PAULA BESSA SANTANA
Externa à Instituição - ELIZABETH MACHADO BARBOSA - UNIFAP