Banca de QUALIFICAÇÃO: ELISA CUNHA PEREIRA

STANDARDIZATION OF CRIOPRESERVATION AND DEFROSTING TECHNIQUES OF CANINE INTESTINAL ORGANOIDS (OIc)

Freezing, cell culture, stem cells, cryoprotectants, culture medium

The intestinal crypts have the capacity in ex vivo to organize and self-regenerate forming structures called intestinal organoids, or "mini guts" Intestinal organoids have great applications as study of regulation, growth and intestinal differentiation, treatment of genetic diseases, evaluation in the therapy of cancer, toxicity analysis of drugs, model of disease studies, substitute in the use of animals for research, etc. However, challenges are still faced within the cell culture laboratories in relation to the maintenance and manipulation of these cells. thawing of canine intestinal organoid lines has been one of the major obstacles due to cell death. In this work, techniques of freezing and decollegation of canine intestinal organolepes will be tested.