Near infrared spectroscopy (NIRS) to estimate "in vitro" and "in situ" enteric methane production
Animal nutrition. Chemical composition. Fecal NIRS. In vitro digestibility.
Methanogenesis. Ruminal Content. Ruminal fluid. Tropical forages. Ruminants.
The ruminant sector contributes to the production of food in the world and these
herbivores, through enteric fermentation, produce methane, which is a potent greenhouse
gas because of its high capacity to absorb infrared radiation. The amount of methane
produced by ruminants can be manipulated and one of the main ways is through feeding, in
the insertion of foods that will decrease methanogenesis, so it is essential to know the
chemical composition, apparent digestibility and methane production potential. Near-
infrared spectroscopy (NIRS) is an alternative technique to conventional methods for
evaluating the nutritional value of foods, since it is a fast method, which does not require
large structures and specific chemical solutions, besides other advantages. The objective of
this study was to do a literature review on the use of NIRS technology in forages, and also
to estimate the in vitro and in situ enteric methane production by the NIRS technique from
two experiments, one aiming to evaluate the in vitro digestibility and methane production
of two tropical forages from different years, also investigating the possibility of using a
calibration model to validate databases of different years and/or species and another one
using male sheep to evaluate digestibility and methane measurement through of the SF6
(sulfur hexafluoride) technique. The spectra of the representative samples of the
experiments, being tropical forages in the first and feces, fluid and ruminal content in the
second, were obtained in the equipment NIRFlex N-500 FT-NIR Spectrometer Technical
Datasheet Cell N500-001. Multivariate analyzes were performed through the NIRCal
software and pre-treatments and treatments, aiming at an adequate adjustment to all the
characteristics and consequent validation of the calibration curves developed with high
robustness. With the present studies, it was concluded that it is possible to obtain
satisfactory calibration and validation parameters in most of the variables related to the
chemical composition and digestibility of forage, so there is no need to develop different
calibration curves per cultivar or year, because from a "global" curve it is possible to
develop robust evaluation models , but in prediction equations of products generated during
the IVDMD, such as methane gas, it is recommended to carry out more conventional
laboratory analysis, aiming to increase accuracy of the models, and from the second
experiment it was concluded that it is possible to predict certain parameters for the
evaluation of methane production, consumption and digestibility by feces being wet or dry
and ground, and the latter presented higher parameters.