Banca de DEFESA: LUCÉLIA MARTINS DE ANDRADE

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : LUCÉLIA MARTINS DE ANDRADE
DATE: 31/03/2025
TIME: 14:00
LOCAL: AUDITÓRIO ISPA
TITLE:

Integrative review of genetic studies in dogs with canine transmissible venereal tumor and expression of the MDR-1 gene and micro-RNAs 126 and 214 in dogs with and without TVT

KEY WORDS:

contagious neoplasm, scientific evidence, resistance gene, mixed type.

PAGES: 41
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Anatomia Patologia Animal
SUMMARY:

Canine transmissible venereal tumor (TVTC) has attracted the interest of researchers due to its dubious origin, resistance to chemotherapy, and the possibility of comparative research in human oncology. In addition, it is one of the tumors that most affects the canine species, with a greater predominance in stray and sexually active animals. This type of tumor has clinical importance due to its transmission capacity. In this sense, we carried out an integrative review (IR) guided by the following guiding question: What is the panorama of genetic studies in dogs with canine transmissible venereal tumor? The following databases were used to search for articles: Pubmed, Scielo, BVS-Vet, and Portal de Periódicos Capes, from 2020 to 2024. We identified 7 articles in Pubmed, 2 articles in Portal de Periódicos Capes, 1 article in BVS-Vet, and no articles were found in Scielo. After analyzing the articles, 8 of the 10 selected articles were classified as level of evidence IV, and 2 articles with level of evidence V. Thus, we concluded that studies with a higher level of evidence are necessary. We also identified that the current scenario of genetic studies in dogs is composed mostly of studies focusing on gene expression, and for this scenario to change, studies that address the different pathways of genetics are essential, so that contributions to the therapy, diagnosis and prognosis of canine transmissible venereal tumor are increased. In addition to the integrative review, we also sought to determine the expression profile of the MDR-1 gene and micro-RNAs 126 and 214 in dogs with and without canine transmissible venereal tumor. In order to do this, samples were collected from 18 dogs, 9 dogs without TVT and 9 with suspected TVT. The diagnosis of dogs with TVT was confirmed based on imprint cytology performed in the Animal Pathology laboratory of UFRA. Biopsy samples from dogs suspected of having TVT were collected from the animal protection project of the Marituba City Hall and from shelters in Benevides and Outeiro. Biopsy samples from dogs in the control group were collected from animals undergoing elective sterilization surgeries at the Zoonosis Control Center, linked to the Belém City Hall. At the Serology and Molecular Biology Laboratory of UFRA, the 18 biopsy samples from the 2 groups underwent ribonucleic acid (RNA) extraction using the Trizol® method, followed by real-time reverse transcription polymerase chain reaction (qRT-PCR). We found that most of the dogs affected by TVT were female, young, and mixed-breed dogs, and the most common cytomorphological type was mixed. We also identified high expression of the MDR-1 gene in dogs with TVT (p<0.05). These results show that high expression of the drug resistance gene, MRD-1, is found in the canine population and may affect treatment, as it requires a greater number of chemotherapy sessions for complete tumor remission.

COMMITTEE MEMBERS:
Presidente - 388501 - WASHINGTON LUIZ ASSUNCAO PEREIRA
Externo ao Programa - 2150577 - ALEXANDRE DO ROSARIO CASSEB - UFRAExterna ao Programa - 1288213 - MARCELLA KATHERYNE MARQUES BERNAL - UFRAExterno ao Programa - 1247731 - THYAGO HABNER DE SOUZA PEREIRA - UFRAExterna à Instituição - GISELLE ALMEIDA COUCEIRO
Externa à Instituição - THAMIRYS ALINE SILVA FARO