Banca de DEFESA: CINTIA LUANA PINHEIRO SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CINTIA LUANA PINHEIRO SANTOS
DATE: 25/02/2022
TIME: 09:00
LOCAL: Plataforma Google Meet
TITLE:

ASSOCIATION OF POLYMORPHISM IN THE 3' UTR OF THE IFN-γ GENE WITH ITS GENE EXPRESSION PATTERN AND miRNA 125b IN THE BLOOD OF DAIRY BUFFALOES WITHOUT HEMOPARASITOSIS.

KEY WORDS:

Buffaloes, genetic improvement, SNP, immune system, IFN-y, miRNA 125b.

PAGES: 71
BIG AREA: Ciências Agrárias
AREA: Zootecnia
SUBÁREA: Genética e Melhoramento dos Animais Domésticos
SUMMARY:

The use of genetic markers as well as the determination of the expression of genes involved in the imune system of buffaloes is extremely valuable in selection programs, they can promote the choice of animals resistant to many pathogens. Thus, the objective of the study was to associate the g4467 G>A polymorphism in the IFNG gene with your gene expression profile as well as miRNA 125b in dairy buffaloes without hemoparasitosis. Blood samples were collected from 145 female buffaloes. DNA extraction was performed through the phenolchloroform method, followed by PCR and RT-PCR techniques for molecular diagnosis of Babesia spp. agents. Trypanosoma spp. and Anaplasma marginale, respectively. Then, all animals were genotyped by the PCR-RFLP technique. Then, RNAs were extracted from animals of different genotypes to determine IFNG mRNA and miRNA expressions through RT-PCR. PCR-RFLP revealed three banding patterns for the studied SNP. Where the genotypes GG, GA and AA were observed, in the proportions of 3.4%, 2.6% and 94.4% respectively and the A allele was the most frequent (95.1%). The SNP showed a deviation from the Hardy-Weinberg Equilibrium (HWE) (P<0.05) and a deficit of heterozygotes with FIS 0.764. All animals of the genotypes found showed expression in both genes, but for the IFNG gene the expression was moderate and for miRNA 125b low. The expression of miRNA 125b showed a significant difference (P<0.05) between the genotypes, with the heterozygote (GA) showing the highest expression. These results suggest that the SNP evaluated in the study population showed low variability because it is a dairy herd that is probably under selection pressure and that healthy animals for hemoparasitosis show the same pattern of IFNG gene expression, but heterozygotes showed greater miRNA125b expression.

BANKING MEMBERS:
Presidente - 1556914 - EDNALDO DA SILVA FILHO
Interno - 1557097 - IGOR GUERREIRO HAMOY
Externo ao Programa - 2150577 - ALEXANDRE DO ROSARIO CASSEB
Externa ao Programa - 2116073 - PRISCILA DI PAULA BESSA SANTANA
Externa à Instituição - ELIZABETH MACHADO BARBOSA - UNIFAP