Banca de QUALIFICAÇÃO: BIANCA BARRETO BARBOSA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : BIANCA BARRETO BARBOSA
DATE: 16/12/2021
TIME: 08:30
LOCAL: https://meet.google.com/wxq-vefa-jyh
TITLE:

Comparison between kinetic vitrification and slow freezing of G. gallus semen: protocols for animal production and conservation of wild bird genetic resources

KEY WORDS:

 

 biotechnology, cryopreservation, spermatozoa, avian reproduction

PAGES: 70
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Inseminação Artificial Animal
SUMMARY:

 

Semen cryopreservation is an important tool for avian conservation programs and management of genetic diversity in rate and endangered species and breeds. In birds, the most commonly used semen conservation method is slow freezing. However during processing, the sperm cell undergoes cryodamage which reduces motility, viability and fertility rates, making it infeasible for both commercial poultry industry and wildlife conservation. Therefore, we aim to evaluate the quality of chicken sperm submitted to slow freezing and kinetic vitrification protocols. For this, a 3-step experiment will be conducted, in which in the first step two devitrification protocols will be evaluated (5ºC for 5 minutes and 42ºC for 10 seconds) and in the second step the maintenance of sperm quality will be evaluated according to different times and trehalose concentrations. In the final step, based on the results of the previous steps, the kinetic vitrification and slow freezing of the samples will be conducted. The parameters evaluated will be motility, morphology, functional viability and integrity of the plasma membrane and mitochondrial activity. It is expected, with the results of this study, to obtain the technological basis for new cryopreservation protocols, that enable the application of these techniques in research laboratories, in industry as well as in avian genetic material conservation of threatened wild species.

BANKING MEMBERS:
Presidente - 480.204.273-68 - SHEYLA FARHAYLDES SOUZA DOMINGUES - UENF
Interno - 1556914 - EDNALDO DA SILVA FILHO
Externo ao Programa - 2853113 - FERNANDO BARBOSA TAVARES
Externa ao Programa - 3160909 - MONICA CALIXTO DA SILVA
Externo à Instituição - MARCEL HENRIQUE BLANK - USP
Externo à Instituição - MARCILIO NICHI - USP
Externa à Instituição - DENISE CALISTO BONGALHARDO - UG