Banca de DEFESA: RAFAELLE CASSEB GUIMARÃES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RAFAELLE CASSEB GUIMARÃES
DATE: 29/11/2024
TIME: 16:30
LOCAL: Google Meet
TITLE:

ASSOCIATION OF AN INDEL FROM THE REGULATORY REGION OF THE MELATONIN RECEPTOR GENE (MTRN1A) WITH MILK PERFORMANCE OF MESTIZED BUFFALOES IN THE AMAZON REGION

KEY WORDS:

 Buffalo farming; Deletion; Breeding.

PAGES: 35
BIG AREA: Ciências Agrárias
AREA: Zootecnia
SUBÁREA: Genética e Melhoramento dos Animais Domésticos
SUMMARY:

Buffalo farming is an important agricultural activity, especially in developing countries and in the Amazon region. These animals have good productive and reproductive performance. Animal breeding programs aim to multiply individuals with characteristics, and it is essential to identify molecular markers of economic importance, which allow the genetic potential of an animal to be determined even before the expression of its phenotype. In this sense, the present study aims to associate a deletion block (INDEL) in the promoter region of the melatonin receptor 1A gene (MTRN1A) with zootechnical data on milk production in a herd of crossbred buffaloes from the Amazon region. For this purpose, blood samples were collected from 70 crossbred buffaloes from a property located in the municipality of Bujaru, state of Pará. Genomic DNA was extracted using the phenolic method. Then, different primers were selected to perform the polymerase chain reactions (PCR), and their products consisted of 162 Pb. They were then sequenced, edited and compared with buffalo reference sequences in the NCBI GenBank . The sequencing results obtained were tabulated and analyzed, determining the allele and genotypic frequencies, observed and expected heterozygosity , inbreeding coefficients (FIS ₎ , Hardy -Weinberg deviation and the probability of Hardy -Weinberg equilibrium. The associations were performed by ANOVA analysis with fixed effect of the genotype and the comparison of means by the Tukey test , through the SAS OnDmand program with a significance level of 0.05%. A total of 70 genotyped animals were observed, at position -1482 of the MTRN1A regulatory region, the genotypes In/In, In/Del and Del/Del. The most frequent allele was In (0.64) and the most frequent genotype was In/Del (0.529). The deviations between the observed and expected genotypes were not significant (P>0.05), thus the population presented Hardy-Weiberg equilibrium . Comparing the zootechnical data of the average daily production of the buffaloes evaluated in the present study, no significant difference was observed in the association with the different genotypes (P>0.05). Thus, the genotypes evaluated had no effect on milk production in buffaloes raised in the Amazon region; however, insertion/deletion polymorphisms can be used in studies of genetic variability, as well as other studies for reproductive characteristics in these animals.

 

COMMITTEE MEMBERS:
Presidente - 1556914 - EDNALDO DA SILVA FILHO
Externa ao Programa - 2116073 - PRISCILA DI PAULA BESSA SANTANA - UFRAExterno à Instituição - CAIO SANTOS SILVA - UFPA
Externo à Instituição - FRED JULIO COSTA MONTEIRO
Externa à Instituição - TAIANARA TOCANTINS GOMES ALMEIDA - UFPA