Banca de DEFESA: MARCELLA KATHERYNE MARQUES BERNAL

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARCELLA KATHERYNE MARQUES BERNAL
DATE: 31/08/2022
TIME: 14:00
LOCAL: Instituto Evandro Chagas. Belém. Auditório do Núcleo de Pesquisa Clínica.
TITLE:

Molecular and histopathological study of Bartonella spp. in liver tissue from rodents and free-living didelphis captured in the State of Pará.

KEY WORDS:

Rodents and neotropical didelphis; Liver; Bartonella spp.

PAGES: 106
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

The host-parasite relationship is characterized as an allelobiotic interaction built through evolutionary-adaptive processes with asymptomatic hosts, in the wild environment the balance of this relationship is notorious, but when there is anthropogenic intervention, an enzootic cycle can start, giving rise to emerging or reemerging diseases. . Among the etiologic agents, Bartonella spp., Is characterized by Gram-negative bacilli of the Proteobacteria class that present tropism by erythrocytes and endothelial cells, with infection already described in animals of the orders: Rodentia, Lagomorpha, Carnivora, Artiodactyla, Eulipotyphla and Chiroptera. The infection by the bacterium may be associated with lymphadenitis, endocarditis, bacillary angiomatosis and liver peliosis in humans. Thirteen species of Bartonella are considered to be zoonotic and, rodents are considered potential reservoirs. And due to the scarcity of studies on the subject and the exponential relevance that this proteobacterium has presented as a disease-causing agent in human and veterinary medicine, research related to the molecular evaluation of this bacterium in mastofaunistic reservoirs may have come to contribute significantly to its eco-epidemiological characterization, genetic variability and identification of possible hosts. The polymerase chain reaction (PCR) has been commonly used in colonies of Bartonella spp. and the study of the polymorphism in the length of the restriction fragment (RFLP) of the genes encoding citrate synthase (gltA) of the RNAr 16S or RNAr 16S-23S regions of the extragenic and random palindromic sequences is used to distinguish strains and species of Bartonellaceae, being an excellent diagnostic method. In this context, the present work aims to carry out the molecular and histopathological study of the occurrence of Bartonella spp. in rodent livers and free-living neotropical didelphis captured in the state of Pará. 184 samples of livers belonging to the Echimyidae, Cricetidae, Muridae and Didelphidae families were analyzed, distributed in 20 genera from four municipalities. In the partial amplification of the gltA gene of the genus Bartonella spp. by PCR to date, 18 positive samples have been registered. Their amplicons were sequenced and the consensus sequences were later aligned with nucleotide sequences representative of the genus Bartonella for phylogenetic analysis. Some histopathological findings of the liver tissue of positive animals were characterized by having a slight diffuse sinusoidal dilation and mononuclear infiltrate.

BANKING MEMBERS:
Interno - 1556914 - EDNALDO DA SILVA FILHO
Interno - 388501 - WASHINGTON LUIZ ASSUNCAO PEREIRA
Externa ao Programa - 2494981 - ADRIANA MACIEL DE CASTRO CARDOSO JAQUES
Externo ao Programa - 2150577 - ALEXANDRE DO ROSARIO CASSEB
Externo à Instituição - DAVID MARCIAL FERNANDEZ CONGA - UFPA
Externo à Instituição - AKIM FELIPE SANTOS NOBRE - UFPA
Externa à Instituição - HELOISA MARCELIANO NUNES - UFPA
Externo à Instituição - PEDRO EDUARDO BONFIM FREITAS - IEC