Banca de DEFESA: JULYNE VIVIAN GUIMARÃES DE CARVALHO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JULYNE VIVIAN GUIMARÃES DE CARVALHO
DATE: 18/02/2022
TIME: 09:00
LOCAL: Plataforma Digital (Google Meet)
TITLE:

VITRIFICATION OF TESTICULAR BIOPSY OF DOMESTIC CAT (Felis catus): IMPROVEMENT OF ASSISTED REPRODUCTION TECHNIQUES FOR ENDANGERED WILD FELINES

KEY WORDS:

Vitrification, Domestic cat, Testicular tissue, cryoprotectants, cryopreservation devices

PAGES: 30
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Reprodução Animal
SPECIALTY: Ginecologia e Andrologia Animal
SUMMARY:

 Testicular biopsy vitrification is a potential assisted reproduction technique to maintain testicular tissue from domestic cats, mainly because it is a viable alternative for the conservation of endangered wild cats. Due to phylogenetic similarity, the domestic cat is accepted as an experimental model for wild feline species. Thereforehe, the objective is to investigate the effect of different associations of permeable and non-permeable cryoprotective agents in vitrification of pubertal domestic cats testicular tissue, as well as determine the most viable vitrification technique for adult cat testicular tissue. The study was divided into two stages. In step 1, the effects caused by the use of 40% ethylene glycol (EG) or the association with dimethyl sulfoxide (DMSO) (20% EG + 20% DMSO), plus 0.1M or 0.5M sucrose or trehalose were evaluated by histological morphology of germ cells, Sertoli cells and measurements of seminiferous tubules analysis after vitrification. In step 2, the vitrification techniques on solid surface (SSV), conventional straws and Ovarian Tissue Cryosystem (OTC) will be evaluated to determine the most viable method to preserve morphological integrity, cell viability and apoptotic index of domestic cat testicular germ cells. In step 1, the preliminary results showed that the Sertoli cells and germ cells have no difference with  the use of the two sugars in the different concentrations at vitrification, however, the morphological integrity was more compromised when the DMSO was used.

BANKING MEMBERS:
Presidente - 480.204.273-68 - SHEYLA FARHAYLDES SOUZA DOMINGUES - UENF
Externa à Instituição - ANDRÉIA MARIA DA SILVA - UFERSA
Externa à Instituição - DANIELLE CRISTINA CALADO DE BRITO - USP
Externa à Instituição - DANUZA LEITE LEAO - UFRA