Banca de QUALIFICAÇÃO: LUCELIA MARTINS DE ANDRADE

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LUCELIA MARTINS DE ANDRADE
DATE: 10/08/2020
TIME: 14:00
LOCAL: Belém
TITLE:

GENE EXPRESSION OF CYTOKINES IN THE BLOOD OF HYPID AND NATURALLY INFECTED FELINE (Felis catus) WITH FELINE HERPESVIRUS TYPE 1

KEY WORDS:

Felis catus; Feline herpesvirus type 1; cytokines; immunology.

PAGES: 15
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

Feline viral rhinotracheitis is caused by feline herpesvirus type 1 and is highly prevalent in the feline population. This study aims to determine the gene expression of cytokines: IL1- β, IL-6, IFN-α, IFN-β, IFN-γ and TNF-α in healthy domestic cats and naturally infected with the virus. Sterile swabs will be collected using nasal, ocular or oropharyngeal secretions and whole blood. Viral DNA will be extracted by the Phenol-chloroform method from secretions collected with swabs, after extraction it will be subjected to a conventional PCR reaction to identify the virus. RNA will be extracted by the Trizol method directly from blood samples and after being quantified it will be used in qPCR reactions in which the cytokine expressions of animal samples will be evaluated with or without viral DNA. Gene expression data will be tested for normality using the Kolmogorov-Smirnov test. Comparisons of gene expressions between positive and negative animals will be determined by Student's t test. The level of significance will be 0.05. All of these analyzes will be carried out in the statistical analyze software (SAS) university edition.

BANKING MEMBERS:
Presidente - 1556914 - EDNALDO DA SILVA FILHO
Interno - 388501 - WASHINGTON LUIZ ASSUNCAO PEREIRA
Externo ao Programa - 1557097 - IGOR GUERREIRO HAMOY
Externa à Instituição - DÉLIA CRISTINA FIGUEIRA AGUIAR - UFPA
Externa à Instituição - Elizabeth Machado Barbosa - UFPA