Banca de QUALIFICAÇÃO: LAURA JAMILLE ARGOLO PAREDES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : LAURA JAMILLE ARGOLO PAREDES
DATE: 30/06/2020
TIME: 14:00
LOCAL: defesa remota por videoconferência
TITLE:

Genetic polymorphisms and expression profiles of genes associated with innate immunity in the ocular conjunctiva in buffaloes in the Amazon Region

KEY WORDS:

Bubalinocultura, melhoramento genético, citocinas inflamatórias, receptores toll-like, defensinas.

PAGES: 43
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Anatomia Patologia Animal
SUMMARY:

The production chain of domestic buffaloes (Bubalus bubalis) has been showing a high performance in recent years, especially in the Brazilian Amazon, although this presents environmental conditions in the tropics that favor the appearance of diseases, including eye infections. Thus, the determination of the immunological status based on evaluations of genes related to innate immunity is necessary in populations of buffalo that inhabit wetlands. In this context, we seek to determine the expression profiles of genes related to innate immunity in the ocular conjunctiva of buffaloes in the Amazon region and to characterize the promoter region of the Interleukin-6 gene, to locate possible SNPs and binding sites of the transcription factors involved in the regulation of expression gene, associating them with resistance or susceptibility to pathogens in these populations. For this, samples of ocular conjunctival tissue and blood were collected from buffaloes slaughtered in the State of Amapá. The tissue fragments were submitted to histological techniques and classified into three groups (G1, G2 and G3) according to the quantitative degree of lymphoid tissue associated with the conjunctiva, in addition to the real-time PCR technique for quantifying the expression of inflammatory cytokine RNA (IL-6, IL-10, TNF-α, IFN-γ), toll-like receptors 4 (TLR-4) and β-defensin-112 (βDEF-112) relative to the endogenous GAPDH gene. The blood was subjected to the conventional PCR method using two pairs of primers from a reference sequence deposited in GenBank 102415101. The PCR products have been purified and will be sequenced, edited and aligned for the analysis of possible polymorphisms to later be associated with the gene expression profiles. In the conjunctiva of the animals of G1 there was no gene expression for most of the studied genes (IL-6, IL-10, TNF-α and βDEF-112), only for IFN-γ and TLR-4. While G2 there was high expression for the IL-6, IL-10, IFN-γ, TLR-4 genes, but not for TNF-α and βDEF-112 and all animals in G3 showed high expression for all the studied genes. The IL-6, IL-10, IFN-γ, βDEF-112 genes showed greater expression in G3 when compared to the other groups. For TNF-α and TLR-4, all groups showed the same level of gene expression. These results may suggest a greater resistance of the G3 buffalo population to pathogenic microorganisms and that there may be a positive correlation between the quantitative degree of lymphoid tissue associated with the conjunctiva and the resistance of individuals.

BANKING MEMBERS:
Presidente - 1556914 - EDNALDO DA SILVA FILHO
Interna - 2313941 - ELANE GUERREIRO GIESE
Externo ao Programa - 2150577 - ALEXANDRE DO ROSARIO CASSEB
Externo ao Programa - 1557097 - IGOR GUERREIRO HAMOY
Externo ao Programa - 2495071 - SEBASTIAO TAVARES ROLIM FILHO
Externa à Instituição - DÉLIA CRISTINA FIGUEIRA AGUIAR - UFPA
Externa à Instituição - Elizabeth Machado Barbosa - UFPA