MOLECULAR IDENTIFICATION OF Bartonella spp. IN THE LIVER TISSUE OF BATS FROM THE BRAZILIAN AMAZON
Bartonella spp. Liver. Neotropical bats.
Bartonellosis is a global vector-borne zoonosis caused by Bartonella, a genus of intracellular Gram-negative bacteria. It is one of 14 neglected emerging infectious diseases that have been recently identified. They infect mammals and are transmitted by arthropod vectors, causing non-specific clinical manifestations. Several Bartonella genotypes have been identified in several wild mammal species, and bats are a significant reservoir for numerous pathogens, including Bartonella spp. Chiropterans are a group of mammals that play a crucial role in the origin and spread of Bartonella bacteria between geographic regions and other groups of mammals. In bats, several taxa of arthropods that feed on blood can be found, which may help in the dispersal of this bacteria. Recognizing the limitations associated with detecting Bartonella species primarily through a single gene and employing only one detection method, conventional PCR was implemented and used to target different genes, allowing for a more nuanced analysis of Bartonella diversity. The present study investigated the occurrence and molecular identity in liver samples (n = 53) from bats of 20 different species from this region, within the Amazon biome and were subjected to molecular detection, to analyze the mutations of these genes, sequencing was carried out of the DNA of two gene regions. This multifaceted approach aimed to provide a more comprehensive understanding of the prevalence of Bartonella species among small mammals in the specified region.