Banca de QUALIFICAÇÃO: LUIS FERNANDO QUEIROZ FARIAS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LUIS FERNANDO QUEIROZ FARIAS
DATE: 28/02/2024
TIME: 14:00
LOCAL: videoconferência VIA GOOGLE MEET
TITLE:

MOLECULAR DETECTION OF Bartonella spp. IN CATS DOMICILED IN THE METROPOLITAN REGION OF MACAPÁ, AMAPÁ

KEY WORDS:

Bartonellosis, felines, State of Amapá, epidemiology

PAGES: 29
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Epidemiologia Animal
SUMMARY:

Bartonellosis is a zoonotic disease caused by intracellular gram-negative bacilli of the genus Bartonella, capable of causing diseases in dogs and cats, and has several species considered zoonotic, highlighting B. hensalae as the main species responsible for causing diseases in humans, even leading to death, and research related to the molecular evaluation of this bacterium in animal reservoirs can contribute significantly to its epidemiological characterization, genetic variability and identification of possible hosts. In this context, the present work aims to carry out a molecular study of the occurrence of infection by Bartonella spp. in cats domiciled in the Metropolitan region of Macapá, State of Amapá. 303 blood samples will be analyzed, collected from 5 veterinary clinics in the study region. Total DNA extraction will be performed using a commercial kit. PCR assays will be developed for partial amplification of the gltA gene from the genus Bartonella spp. (350 bp). The amplicons from positive samples will be purified and sequenced in the forward and reverse directions, the consensus sequences will be obtained using the BioEdit software (v7.2.6) and submitted to the BLAST platform. Then, aligned with a database containing representative sequences of the 45 species of the genus available in the Genbank database. For phylogenetic analysis, the Maximum-Likelihood method and the Kimura 2-parameter model will be used in the MEGA v7.0 software, for taxonomic identification of the isolates. The nucleotide and amino acid distance will be calculated using the Geneious v8.1.3 software.

BANKING MEMBERS:
Presidente - 388501 - WASHINGTON LUIZ ASSUNCAO PEREIRA
Interno - 1556914 - EDNALDO DA SILVA FILHO
Externa ao Programa - 2494981 - ADRIANA MACIEL DE CASTRO CARDOSO JAQUES
Externo à Instituição - FRED JULIO COSTA MONTEIRO
Externa à Instituição - DÉLIA CRISTINA FIGUEIRA AGUIAR - UFPA