Banca de QUALIFICAÇÃO: FLÁVIA LETÍCIA GURJÃO DE ANDRADE

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : FLÁVIA LETÍCIA GURJÃO DE ANDRADE
DATE: 27/02/2024
TIME: 10:00
LOCAL: Sala virtual - Plataforma Google meet
TITLE:

ASPECTOS MORFOLOGICOS E MOLECULARES DOS PARASITOS DE PEIXES ORIUNDO DA MICROREGIÃO DO SALGADO, NA ILHA DE COLARES/PA

KEY WORDS:

Myxozoa, Parasite, Colares, Brazil

PAGES: 50
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Fish serve as hosts to various parasites, including those of the Myxozoa Class (phylum Cnidaria), such as the genus Kudoa, which parasitize marine and estuarine fish, and are rarely found in freshwater. They cause lesions in the skeletal musculature of the host, rendering the flesh unfit for consumption after death. Another group, the genus Ellipsomyxa, belonging to the Bivalvulida class in the Ceratomyxidae family, represents a small group that infects the gallbladder of fish and belongs to another phylum, Apicomplexa. The genus Calyptospora, within the same phylum, parasitizes the liver parenchyma of fish. Micro-parasites are commonly studied for their negative effects on commercial fishing. The fish Cathorops spixii, popularly known as uricica, is a catfish of the Ariidae family, with low commercial value but widely consumed by the riverine population, as well as Hypophthalmus marginatus, commonly known as mapará, and Ageneiosus inermis, commonly known as mandubé. This study identified the presence of micro-parasites infecting specimens of Cathorops spixii, Hypophthalmus marginatus, and Ageneiosus inermis, collected on Humaitá Beach, in the municipality of Colares/PA, from January to March 2023. The methodology included necropsies on 30 individuals of each species, aiming to detect micro-parasites. When material was identified, it was collected for analysis through light microscopy and molecular biology. Cysts were observed in 80% of the examined animals. During necropsy, spores morphologically similar to Kudoa sp., Ellipsomyxa sp., and Calyptospora sp., were identified in the fish. Histopathological analyses revealed significant alterations in the muscle tissue of Cathorops spixii, indicating the presence of the genus Kudoa. Molecular biology analyses for Cathorops spixii yielded results with partial sequences of 971 base pairs indicating the presence of Kudoa and 960 base pairs indicating the presence of Calyptospora. For Hypophthalmus marginatus and Ageneiosus inermis, the results were 897 and 912 base pairs, respectively, indicating the presence of Ellipsomyxa sp. All sequences were from the SSU rDNA gene and were sequenced and compared with reference sequences in GenBank. The collections were approved by the Animal Use Committee of the Federal Rural University of the Amazon with registration in the Biodiversity Authorization and Information System. This study aims to verify the occurrence of micro-parasites in the fishes uricica, mapará, and mandubé, using light microscopy and molecular biology techniques, characterize the morphology of the found micro-parasites, and obtain 18S rDNA gene sequences of these parasites. The analyses indicated the possible existence of a new Kudoa species, distinguished by distinct characteristics from other Myxozoa, emphasizing the importance of this parasite in causing damage to the musculature of Cathorops spixii, which plays a significant role in the teleost fauna in the Amazon, as well as the other studied fishes.

BANKING MEMBERS:
Externo à Instituição - DIEHGO TULOZA DA SILVA
Presidente - 1557097 - IGOR GUERREIRO HAMOY
Externo à Instituição - MARCELO FRANCISCO DA SILVA
Externo à Instituição - SÁVIO LUCAS DE MATOS GUERREIRO