Banca de QUALIFICAÇÃO: PAULO DE TARSO SANTANA TAVARES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : PAULO DE TARSO SANTANA TAVARES
DATE: 10/10/2023
TIME: 14:00
LOCAL: Sala Virtual
TITLE:

Molecular diagnosis of Toxoplasma gondii in free-range chickens (Gallus gallus) sold at fairs in the metropolitan region of Macapá - AP

KEY WORDS:

Toxoplasmosis, PCR, Diagnosis, Gallus gallus

PAGES: 30
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Toxoplasmosis is an infectious disease caused by the parasite Toxoplasma gondi, which can affect
humans and animals. Contamination occurs mainly by eating food contaminated with the parasite,
such as raw or undercooked meat, or by contact with feces of infected cats. Although it is a
common disease worldwide, epidemiological studies show that its prevalence varies according to
environmental, behavioral and cultural factors. Among the species of animals that can be affected
by toxoplasmosis are birds. In many regions of the world, chickens are raised in precarious living
conditions, with limited access to adequate hygiene and food, which can favor the spread of the
parasite. In addition, the sale of chickens in free markets is a common practice in many cities, and
the sanitary quality of these animals cannot be guaranteed. The objective of this work is to identify
the occurrence of T.gondi in free-range chickens in the metropolitan region of Macapá, through
molecular tests PCR (Polymerase Chains Reaction) and to analyze the genetic sequencing of the
DNA of T.gondi from positive birds. 32 slaughtered birds will be purchased from 8(eight) fairs in
the metropolitan region of Macapá, with 4(four) birds per fair. From each bird, 3 heart samples will
be collected, totaling 192 molecular reactions. These will be condition and processed at the
Laboratory of Microbiology/Immunology (BIOMOL), at the Department of Medicine at UNIFAP
(Federal University of Amapá). Genetic sequencing of T.gondi positive samples will be performed
using the NCBI (National Center for Biotechnology Information) identification program. The data
will be allocated in an electronic spreadsheet to carry out statistical analysis and observation of
positive birds according to the free fairs located.

BANKING MEMBERS:
Interno - 388501 - WASHINGTON LUIZ ASSUNCAO PEREIRA
Externa ao Programa - 2116073 - PRISCILA DI PAULA BESSA SANTANA
Externo à Instituição - RICARDO MARCELO DOS ANJOS FERREIRA - UNIFAP
Externa à Instituição - ELIZABETH MACHADO BARBOSA - UNIFAP
Externo à Instituição - LAILSON DO NASCIMENTO LEMOS - UNIFAP