Banca de DEFESA: ANA CAROLINA DE BARROS MOURA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANA CAROLINA DE BARROS MOURA
DATE: 31/07/2023
TIME: 09:00
LOCAL: Plataforma virtual Google meet
TITLE:

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KEY WORDS:

Trypanosoma vivax; Anaplasma marginale; LAMP; real-time PCR; PCR; molecular diagnosis

PAGES: 54
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Epidemiologia Animal
SUMMARY:

The morbidity and mortality due to diseases caused by hemoparasites such as anaplasmosis and trypanosomosis still represent today severe damage to livestock and a relevant challenge to scientific research on animal health. These hemoparasitoses are characterized by a silent course and low parasitemia, making diagnosis difficult. Moreover, when present, the symptoms are nonspecific, favoring the improper administration of veterinary drugs that result in treatment inefficiency and increased drug resistance. Evaluating the quality of diagnostic tests is a topic of interest in clinical and epidemiological research since proper treatment of a disease begins based on rapid and correct pathogen detection or disease diagnosis. More recently, the LAMP diagnostic technique has proven to be more sensitive than PCR, highly specific, and with minimal laboratory structure requirements. This study aimed at comparatively analyzing the performance of PCR, real-time PCR and LAMP techniques in the detection of Anaplasma marginale and Trypanosoma vivax in naturally infected cattle and buffaloes in Amapá state, Brazilian Amazon. A total of 150 whole blood samples were analyzed at the UFRA serology and molecular biology laboratory. The study confirmed the circulation of T. vivax protozoan and A.marginale bacteria in Amapá herds, predominantly asymptomatically. However, the kappa statistical test did not show satisfactory agreement between the techniques used. The prevalence rates obtained by conventional PCR were the lowest (4% for T. vivax and 3,3% for A. marginale), suggesting that this test is not recommended for use in screening herds. For T. vivax and A. marginale, respectively, real-time PCR expressed 38% and 28.6% detection and the LAMP technique obtained 83.3% and 56% positive samples. The LAMP test revealed superior sensitivity in the detection of these hemoparasites, demonstrating to be a potential alternative for the clinical and epidemiological molecular diagnosis of animal anaplasmosis and trypanosomosis in animals with low parasitemia from endemic regions. Further studies should be carried out to adjust the LAMP protocols for their adaptation to simpler laboratories close to the rural environment.

BANKING MEMBERS:
Interno - 388501 - WASHINGTON LUIZ ASSUNCAO PEREIRA
Interno - 1557097 - IGOR GUERREIRO HAMOY
Externo ao Programa - 388484 - RAIMUNDO NONATO MORAES BENIGNO
Externa ao Programa - 2116073 - PRISCILA DI PAULA BESSA SANTANA
Externo ao Programa - 2495071 - SEBASTIAO TAVARES ROLIM FILHO
Externa à Instituição - LIVIA MEDEIROS NEVES CASSEB - UFPA
Externo à Instituição - SANDRO PATROCA DA SILVA - NENHUMA