Banca de DEFESA: ANA CAROLINA DE BARROS MOURA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANA CAROLINA DE BARROS MOURA
DATE: 31/07/2023
TIME: 09:00
LOCAL: Plataforma virtual Google meet
TITLE:

Evaluation of the LAMP technique as an alternative molecular diagnostic method to study the prevalence of hemoparasites in herds in Amapá

KEY WORDS:

hemoparasites; LAMP; real-time PCR; conventional PCR; diagnosis; buffalo.

PAGES: 50
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Epidemiologia Animal
SUMMARY:

Morbidity and mortality due to the diseases of parasitic sadness for scientific research and trypanosso still represent a challenge to public and relevant animal health today. In addition, the symptomatic non-specificity of these diseases can favor the administration of veterinary drugs aiming at the inefficiency of treatments and drugs, affecting the quality and safety of the product that reaches the consumer. The evaluation of the quality of diagnostic tests is a topic of interest in clinical and epidemiological research as the adequate treatment of a disease starts with the rapid and correct detection of pathogens or diagnosis of the disease. More recently, a diagnostic technique LAMP has been shown to be more sensitive than PCR, highly specific and requiring minimal laboratory structure. In this perspective, it is believed that the LAMP technique may be a viable, early and effective alternative for routine use and monitoring of hemoparasites, capable of expanding access and reducing the limitations of traditional molecular diagnoses. Therefore, the objective of this study is to perform a comparative analysis between conventional PCR, real-time PCR and LAMP techniques for the diagnosis of natural infection by Babesia bovis, Babesia bigemina, Anaplasma marginale and Trypanosoma vivax, evaluating sensitivity, specificity, applicability and cost-effectiveness of each method. Will be analyzed 150 samples of whole blood collected from cattle and buffaloes raised on farms in the municipalities of Santana and Mazagão, State of Amapá. Thus, it is expected to detect infected animals by these hemoparasites, identifying agreement in the results obtained by the three techniques applied and proving the LAMP assay as a viable alternative for molecular diagnosis considering its specificity, sensitivity, low cost and field applicability.

BANKING MEMBERS:
Interno - 1557097 - IGOR GUERREIRO HAMOY
Externa à Instituição - LIVIA MEDEIROS NEVES CASSEB - UFPA
Externa ao Programa - 2116073 - PRISCILA DI PAULA BESSA SANTANA
Externo ao Programa - 388484 - RAIMUNDO NONATO MORAES BENIGNO
Externo à Instituição - SANDRO PATROCA DA SILVA - NENHUMA
Externo ao Programa - 2495071 - SEBASTIAO TAVARES ROLIM FILHO
Interno - 388501 - WASHINGTON LUIZ ASSUNCAO PEREIRA