Use of biochar as a mitigator in response to carbon and nitrogen metabolism in pau pretinho (Cenostigma tocantinum) seedlings submitted to nickel doses
Phytoremediation; Heavy metal; Attenuator; Metabolism
The use of plants with phytoremediation potential is a practice used in soils contaminated by
heavy metals, which are environmental pollutants and their toxicity is a problem of worldwide
importance. The species Pau-pretinho (Cenostigma tocantinum Ducke) will be used in terms
of its capacity to tolerate this type of stress subjected to doses of the heavy metal nickel
chloride (NiCl2) and as a mitigator the biochar of açaí seed (Euterpe oleracea Mart). In this
study, the growth and biochemical metabolism of carbon and nitrogen (reducing and non-
reducing sugars, total soluble carbohydrates, sucrose, amino acids, proteins, proline, glycine
and betaine, free ammonium, nitrate reductase) in seedlings of Pau-pretinho. The experiment
is being conducted in a greenhouse, in an area of the Institute of Agricultural Sciences (ICA),
belonging to the Federal Rural University of the Amazon - UFRA, campus Belém-PA. The
design will be completely randomized (DIC) 4 x 3 and consisting of 5 repetitions. Nickel
chloride doses (NiCl2 0, NiCl2 70mg/kg; NiCl2 100mg/kg; NiCl2 130mg/kg) and biochar
doses (0%; 3.5%; 7%) using 60 seedlings in total. As a result, it is expected that the studied
species has a phytoremediating potential in relation to nickel and that biochar has a mitigating
effect on carbon and nitrogen metabolism and promotes greater tolerance to nickel.