Banca de DEFESA: DANYLLO AMARAL DE OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : DANYLLO AMARAL DE OLIVEIRA
DATA : 25/02/2019
HORA: 09:00
LOCAL: Auditorio da Biblioteca da UFRA
TÍTULO:

Characterization of endophytic Pseudomonas regarding to the production of potentially biostimulating compounds of plant development

PALAVRAS-CHAVES:

PGPB, Indole acetic acid, Siderophores, Phosphate solubilization

PÁGINAS: 50
GRANDE ÁREA: Ciências Agrárias
ÁREA: Agronomia
SUBÁREA: Fitossanidade
ESPECIALIDADE: Microbiologia Agrícola
RESUMO:

Pseudomonas is a genus of gram-negative bacteria frequently found in soil or in symbiotic relationship with plants. When these bacteria occur into internal tissues of plants, they are denominated endophytic bacteria. Among them, some bacteria are known as Plant Growth-Promoting Bacteria (PGPB), since they are able to produce phytostimulants, biofertilizers and biocontrol substances. Previous studies have identified Pseudomonas bacteria (isolates Pt12 and Pt13) associated with roots of Piper tuberculatum, a piperaceae occurring in the Amazon region with known resistance to infection by Fusarium solani f. sp. piperis, causal agent of fusariosis of  black pepper (Piper nigrum L.). The present study aims to characterize these two isolates regarding their abilities to produce siderophores and indoleacetic acid (AIA) and solubilize inorganic phosphates, as well as to detect DNA sequences of proteins potentially related to biosynthetic pathways of these substances. Our results showed that isolates Pt12 and Pt13 were able to produce AIA, especially in the presence of high concentration of tryptophan, indicating that they are IAA-producing bacteria by tryptophan-dependent biosynthetic pathways. The isolate Pt12, previously identified as a Pseudomonas putida, was also able to produce siderophores when cultivated on LB (Luria-Bertani) and TS (Tryptone Soybean) media, and analyzed through CAS (Chrome Azurol S) assay. In addition, when cultivated in NBRIP medium the isolate Pt12 was able to solubilize tri-calcium phosphate, which was evidenced by detection of transparent halos around the bacterial colonies.

MEMBROS DA BANCA:
Presidente - 409.858.651-72 - CLAUDIA REGINA BATISTA DE SOUZA - UFPA
Externo ao Programa - 3065086 - GLEDSON LUIZ SALGADO DE CASTRO
Externo ao Programa - 2935820 - ALINE MEDEIROS LIMA
Externo à Instituição - AGENOR VALADARES SANTOS - UFPA