NUTRIENTS THAT INFLUENCE PILOCARPINE PRODUCTION IN JABORANDI PLANTS
Nutrients. P. microphyllus. Secondary metabolite.
Species of the genus Pilocarpus, popularly known as Jaborandi, are plants that produce the substance pilocarpine (alkaloid), used in the pharmaceutical industry. In Pará, the species Pilocarpus microphyllus is the most important, as it has the highest concentration of pilocarpine, and can be found near the Carajás National Forest (FLONA). Its cultivation in planted areas is still complex, since the main difficulty is to understand the factors that favor the greater production of this substance, also permeating nutritional aspects that are still unclear. In this context, this research aims to evaluate the nutrients that influence pilocarpine production in jaborandi plants. For this, two surveys were carried out, in the first one collection of P. microphyllus plants and soil in areas of the National Forest of Carajás were carried out, where the chemical parameters of the soil and levels of foliar nutrients were correlated with the production of pilocarpine. And in the second test, jaborandi plants were submitted to five doses of N (0; 90; 180; 270 and 360 mg/kg-1 of N), with ten repetitions. The characteristics evaluated were biomass, pilocarpine content, gas exchange and nutrient content (N, P, K, Ca, Mg, S, Fe, Mn, Zn and Cu). The results found in the first study show that through multivariate analysis, the model that best predicted pilocarpine production was composed of leaf N, leaf Mg, soil Fe (positive predictors), soil S and leaf P (negative predictors). As for the effects of nitrogen doses, the results indicate that the highest nitrogen input led to a continuous gain in biomass yield, greater nutrient absorption (Fe, Zn and N), and even though many gas exchange parameters have been altered, jaborandi plants were able to maintain metabolic processes. The presence of pilocarpine was detected in all parts of the plant, highlighting the leaf tissue with the highest amount of pilocarpine, suggesting a possible biosynthetic route, initiated in the root, being stimulated by the marked presence of iron and root nitrogen.