ACTION OF BRASSINOSTEROIDS ON ANATOMIC, STRUCTURAL, PHYSIOLOGICAL, BIOCHEMICAL, NUTRITIONAL AND GROWTH CHARACTERISTICS IN SOYBEAN PLANTS [(Glycine max (L.) Merr.] UNDER NICKEL TOXICITY
High dose of Ni, 24- Epibrassinolide, Glycine max, Anatomical Root.
Soybean [Glycine max (L.) Merrill] is a legume of great socioeconomic relevance, both nationally and internationally. Excessive Ni can seriously affect germination, plant growth and impair enzyme activity. EBRs are presented as a new group of phytohormone steroids that act to stimulate a multitude of physiological reactions in the plant including cellular and metabolic functions. Our objective was to reveal the structural, physiological, biochemical, nutritional and growth behavior of soybean subjected to stress by high dose of Ni and to identify the possible benefits caused by EBR. The experiment was randomized with four treatments, including two concentrations of Ni (0 and 200 µM Ni, described as - Ni2 + and + Ni2 +, respectively) and two concentrations of 24-epibrassinolide (0 and 100 nM EBR, described as - EBR and + EBR, respectively. The excess of Ni2 + caused damage to the root and leaf structures, causing anatomical changes in these tissues. At the root, the EBR increased the epidermis (27%), protecting the root from the Ni2 + ion. , significant increases in palisade (11%) and spongy parenchyma (29%). the plants submitted to EBR and Ni dispensed increments in comparison with the treatment without EBR of 50%, 27%, 40% SOD, CAT, APX and POX and 19%, respectively, compared to treatment without EBR Our results confirm that the pretreatment with 100 nM of EBR clearly mitigated the anatomical disturbances caused by the excess of Ni in the structures of the leaves and roots with positive performance also in the attenuation of high nickel dosage in nutritional, physiological, biochemical and growth characteristics in soybean.