Brassinosteroids positively modulate growth: Physiological, biochemical and anatomical evidences using two tomato genotypes contrasting to dwarfism
Chlorophyll fluorescence ● Dwarf gene ● Gas exchange ● Leaf anatomy ● Solanum lycopersicum ● 24-epibrassinolide
Growth and development are vital processes in the life cycles of plants. Brassinosteroids (BRs) are steroids that when exogenously applied can regulate several biological responses. The aim of this research was to investigate the possible interferences caused by the exogenous application of BR on growth and metabolism using two genotypes of the Dwarf gene, MT-d and MT-D, that are BR-deficient and BR-efficient, respectively. The experiment had four treatments with two genotypes (BR-efficient and BR-deficient) and two levels of brassinosteroids (0 and 100 nM BR, described as – BR and + BR, respectively). This study revealed that the exogenous application of BR promoted improvement in growth, inducing increases in all variables. The positive effects on gas exchange and chlorophyll fluorescence confirm the benefits of this steroid on the photosynthetic apparatus. The changes in the anatomical characteristics of the leaf are related to the contribution of BR on the influx and consequent fixation of CO2. In addition, modifications related to root anatomy, occurring by the BR action, suggest an increase in the efficiency of the absorption of water and nutrients. Increases in photosynthetic pigments suggest that the role of BR is linked with chlorophyll biosynthesis and the maintenance of chloroplast integrity, resulting from associations with the increases found in the activities of antioxidant enzymes that modulate the accumulation of reactive oxygen species.